Gamma Interferon Production by Different Populations of Human Splenic Lymphocytes
- 1 January 1983
- journal article
- Published by Mary Ann Liebert Inc in Journal of Interferon Research
- Vol. 3 (1) , 121-128
- https://doi.org/10.1089/jir.1983.3.121
Abstract
The treatment of cultures of human splenic lymphocytes with Staphylococcal enterotoxin A (SEA) resulted in the production of human gamma interferon (HuIFN-gamma). Separation of lymphocyte populations to give preparations enriched in T or B lymphocytes after removal of macrophages demonstrated that maximum IFN titers were detected when T lymphocytes were incubated with macrophages for a period of three days. The levels of IFN-gamma varied from donor to donor but the kinetics of induction were quite similar in all cases. Concentrations of 0.5-1.0 microgram/ml SEA gave optimal induction of IFN and the addition of thymosin alpha-1 at 10 micrograms/ml to the culture medium enhanced IFN-gamma production.Keywords
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