PREPARATION AND CHARACTERIZATION OF THE ANTI-IDIOTYPIC PROPERTIES OF RABBIT ANTI-TIMOTHY ANTIGEN-B HELPER FACTOR AND ANTI-MOUSE TIMOTHY IGE ANTISERA

  • 1 January 1982
    • journal article
    • research article
    • Vol. 45  (2) , 217-225
Abstract
Rabbit anti-T-helper factor (anti-THF) was prepared by immunization with affinity-purified timothy antigen B (AgB)-speceific helper factor. The rabbit anti-THF anti-THF and an anti-IgE fraction (anti-Eid) purified by a Sepharose-AgB-timothy IgE affinity adsorbent were evaluated for the presence of antiidiotypic antibodies. Both antibodies (anti-THF and anti-Eid) specifically induce [3H]-thymidine incorporation of AgB-primed T cells at optimum concentrations specifically block antigen-induced lymphocyte proliferation when preincubated with AgB-primed T cells at an excess concentration and specifically stimulate in vivo IgE formation when preincubated with AgB-primed B or T cells and injected into syngeneic X-irradiated recipients. Anti-THF and anti-Eid purified by an AgB-specific T suppressor factor (TSF) affi-gel adsorbent retain their ability to specifically initiate [3H]-thymidine incorporation of AgB-primed T cells. Thus, both anti-THF and anti-Eid recognize unique determinants present on AgB-specific T-helper, T-suppressor and B cells, and the receptors on AgB-specific T and B cells apparently share cross-reactive idiotypic determinants.

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