A COMPARATIVE STUDY OF SUPPORTS FOR THE SYNTHESIS OF OLIGONUCLEOTIDES WITHOUT USING AMMONIA
- 1 November 1996
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 15 (11-12) , 1871-1889
- https://doi.org/10.1080/07328319608002739
Abstract
A comparative study of the cleavage efficiency of succinyl, phthaloyl, oxalyl, 2-(2-nitrophenyl)ethyl, 9-fluorenylmethyl, and 2-nitrobenzyl supports in 0.5M DBU solutions is described. A decrease in cleavage efficiency is observed when small oligonucleotides containing thymidine are linked to the supports. In these conditions oxalyl supports gave the best yields followed by 2-(2-nitrophenyl)ethyl and 9-fluorenylmethyl supports.Keywords
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