A protein kinase C‐related enzyme activity in Dictyostelium discoideum
- 14 August 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 253 (1-2) , 71-75
- https://doi.org/10.1016/0014-5793(89)80932-9
Abstract
In crude cell lysates of the cellular slime mould Dictyostelium discoideum we identified a protein kinase C (PKC)‐like enzyme activity. This activity, measured as phosphorylation of a synthetic EGF‐receptor‐derived peptide [(1987) J. Biol. Chem. 262, 772–777], was regulated by Ca2+, phosphatidylserine (PS), 1,2‐dioleoyl‐rac‐glycerol (DG) and the phorbol ester PMA. PS and DG stimulated the enzyme in a synergistic manner. The stimulation by these lipids was, in contrast to what has been found for ‘classical’ mammalian PKC, not dependent on Ca2+. The D. discoideum enzyme was strongly stimulated by nanomolar concentrations of PMA, and inhibited by PKC‐inhibitor staurosporine.Keywords
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