Salmonella typhimurium gyrA mutations associated with fluoroquinolone resistance
- 1 July 1995
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 39 (7) , 1621-1623
- https://doi.org/10.1128/aac.39.7.1621
Abstract
Spontaneous quinolone-resistant mutants obtained from Salmonella typhimurium Su694 were screened for mutations by direct DNA sequencing of an amplified PCR gyrA fragment. Substitutions Ser-83-->Phe (Ser83Phe), Ser83Tyr, Asp87Tyr, and Asp87Asn and double mutation Ala67Pro-Gly81Ser, which resulted in decreased sensitivities to ciprofloxacin, enoxacin, pefloxacin, norfloxacin, ofloxacin, and nalidixic acid, were found. The levels of resistance to quinolones for each mutant were determined.Keywords
This publication has 37 references indexed in Scilit:
- Amplification and nucleotide sequence of the quinolone resistance-determining region in thegyrAgene of mycobacteriaFEMS Microbiology Letters, 1994
- On the mechanism of action of quinolone drugsTrends in Microbiology, 1993
- High-level fluoroquinolone resistance in a Salmonella typhimurium isolate due to alterations in both gyrA and gyrB genesJournal of Antimicrobial Chemotherapy, 1993
- In-vitro selection of fluoroquinolone resistance in Salmonella typhimuriumJournal of Antimicrobial Chemotherapy, 1993
- The molecular basis of quinolone actionJournal of Antimicrobial Chemotherapy, 1992
- Detection of gyrA gene mutations associated with ciprofloxacin resistance in methicillin-resistant Staphylococcus aureus: analysis by polymerase chain reaction and automated direct DNA sequencingAntimicrobial Agents and Chemotherapy, 1992
- Control of bacterial DNA supercoilingMolecular Microbiology, 1992
- Mycoplasma pneumoniae DNA gyrase genesMolecular Microbiology, 1990
- Inhibitors of DNA gyrasePharmacology & Therapeutics, 1989
- Cloning and sequencing of the Escherichia coli gyrA gene coding for the a subunit of DNA gyraseJournal of Molecular Biology, 1987