Vasopressin stimulates prostaglandin and thromboxane synthesis in toad bladder epithelial cells

Publisher Website
Google Scholar
Abstract
The effects of arginine vasopressin (AVP) and its nonpressor analog desamino-8-D-arginine vasopressin (dDAVP) on immunoreactive prostaglandin [PG] E and thromboxane [Tx] B2 synthesis from endogenous arachidonic acid by epithelial cells isolated from toad urinary bladders were investigated. In epithelial cell suspensions prepared using a collagenase treatment, AVP (5 mU/ml) stimulated PG E synthesis from 0.27 .+-. 0.05 to 0.53 .+-. 0.09 pmol .cntdot. min-1 .cntdot. mg protein-1 (P < 0.01, n = 6) and stimulated Tx A2 synthesis, as assessed by measurement of its stable metabolite Tx B2, from 0.032 .+-. 0.004 to 0.054 .+-. 0.009 pmol .cntdot. min-1 .cntdot. mg protein-1 (P < 0.02, n = 6). dDAVP (130 nM) also stimulated immunoreactive PG E and Tx B2 synthesis (P < 0.05, n = 6). In cells prepared by EDTA treatment or scraping, AVP did not alter PG or Tx synthesis. AVP and dDAVP stimulate both PG and Tx synthesis in epithelial cells from the toad urinary bladder. Evidently, the antidiuretic activity of these peptides is associated with this effect. The results are consistent with previous observations that the synthesis of PG E and Tx by epithelial cells results in a negative and positive modulation, respectively, of the action of AVP on water transport.