Light-absorbing and Scattering Properties of Nonhaemolysed Blood

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Abstract
Light transmission by thin films of nonhaemolysed blood is affected by the multiple scattering that occurs in undiluted blood. Previous studies did not permit investigation in terms of a light-scattering theory. The present study was made with an integrating-sphere spectrophotometer. Application of Twersky's theory for the multiple scattering of waves permitted separation of the effects of absorption and scattering on the light transmittance of nonhaemolysed blood. It is shown that the relationship between light scattering and red-cell concentration is parabolic and that the absorption of light within the erythrocyte is the same as in a haemoglobin solution.