Molecular characterization of the kinetoplastid membrane protein-11 genes from the parasiteTrypanosoma rangeli

3 February 2005

journal article
research article
Published by Cambridge University Press (CUP) in Parasitology

Vol. 130 (6) , 643-651
https://doi.org/10.1017/s0031182004006936

Abstract

Trypanosomatids are early divergent parasites which include several species of medical interest.Trypanosoma rangeliis not pathogenic for humans but shows a high immunological cross-reactivity withTrypanosoma cruzi, the causative agent of Chagas' disease that affects more than 17 million people throughout the world. Recent studies have suggested thatT. cruziKMP-11 antigen could be a good candidate for the induction of immunoprotective cytotoxic responses againstT. cruzinatural infection. In the present paper the genes coding for theT. rangelikinetoplastid membrane protein-11 have been characterized. The results show that the locus encoding this protein is formed by 4 gene units measuring 550 nucleotides in length, organized in tandem, and located in different chromosomes in KP1(+) and KP1(−) strains. The gene units are transcribed as a single mRNA of 530 nucleotides in length. Alignment of theT. rangeliKMP-11 deduced amino acid sequence with the homologous KMP-11 protein fromT. cruzirevealed an identity of 97%. Interestingly, the T and B cell epitopes of theT. cruziKMP-11 protein are conserved in theT. rangeliKMP-11 amino acid sequence.

Keywords

This publication has 44 references indexed in Scilit:

Acute Trypanosoma cruzi infection: IL-12, IL-18, TNF, sTNFR and NO in T. rangeli-vaccinated mice
Vaccine, 2003
Trypanosoma cruzi heat‐shock protein‐70 kDa, alone or fused to the parasite KMP11 antigen, induces functional maturation of murine dendritic cells
Immunology & Cell Biology, 2002
Attenuated Toxoplasma gondii ts-4 mutants engineered to express the Leishmania antigen KMP-11 elicit a specific immune response in BALB/c mice
Vaccine, 2001
DNA Immunization withTrypanosoma cruziHSP70 Fused to the KMP11 Protein Elicits a Cytotoxic and Humoral Immune Response against the Antigen and Leads to Protection
Infection and Immunity, 2001
The immunization of A2/Kb transgenic mice with the KMP11-HSP70 fusion protein induces CTL response against human cells expressing the T. cruzi KMP11 antigen: identification of A2-restricted epitopes
Molecular Immunology, 2001
Molecular karyotype and chromosomal localization of genes encoding ß-tubulin, cysteine proteinase, hsp 70 and actin in Trypanosoma rangeli
Memórias do Instituto Oswaldo Cruz, 2001
The humoral immune response to the kinetoplastid membrane protein-11 in patients with American Leishmaniasis and Chagas disease: prevalence of IgG subclasses and mapping of epitopes
Immunology Letters, 2000
Molecular Characterization of KMP11 fromTrypanosoma cruzi: A Cytoskeleton-Associated Protein Regulated at the Translational Level
DNA and Cell Biology, 2000
Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11
Memórias do Instituto Oswaldo Cruz, 1998
Electrophoretic Karyotype and Linkage Groups of the Amoeboflagellate Naegleria gruberi
The Journal of Protozoology, 1990