Measurement of mean corpuscular and packed cell volumes with a Coulter cell counter.

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Abstract
The most valuable features of the computer attachments were the saving of time normally required to obtain the PCV [packed cell volume] and MCV [mean corpuscular volume] and the knowledge that the computer result of the MCV was independent of errors often associated with 2 sequential dilution processes. The mean cell volume was previously compared with the median cell volume derived from the median threshold on a model A cell counter (standard deviation of the difference 3.8 cu[center dot][mu]) and the standard deviation of the computer attachment was no different (SD =4.1 cu[center dot][mu]). However, unlike previous experience in comparing the median and mean cell volumes, there were no examples of consistent differences between the 2 methods of computing the MCV and it seems probable that the difference is almost wholly due to errors in reading the microhematocrit and preparing 2 sequential dilutions for the red cell count The PCV recorded by the Coulter attachment is dilution dependent, but the errors associated do not seem any greater than those of reading a microhematocrit.

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