DNA synthesis is initiated at two positions within the origin of replication of plasmid R1162
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (20) , 8319-8331
- https://doi.org/10.1093/nar/15.20.8319
Abstract
DNA synthesis of broad host-range plasmid R1162 is initiated from two positions, flanking a large (40 bp sten, 40 bp loop) inverted repeat. Each start-point is located within a highly conserved, but oppositely oriented, 10 base-pair sequence. Synthesis from the two positions converges within the intervening inverted repeat. An analysis of deletions suggests that both start positions must be present for synthesis. A model describing possible early events in replication of plasmid R1162 is presented.Keywords
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