Propagation and Modification of Infectious Bovine Rhinotracheitis (IBR) Virus in Porcine Kidney Tissue Culture

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Abstract
IBR virus was isolated in porcine kidney tissue culture from infected respiratory tissues of cattle and produced a typical cytopathogenic effect on these cells. This virus was passed serially in porcine kidney tissue culture and, when inoculated intranasally into susceptible cattle, produced signs characteristic of IBR. IBR virus passed previously through 7 passages in bovine kidney tissue culture could also be successfully propagated in porcine kidney tissue culture and in two separate attempts was passed 60 and 100 times, respectively, in porcine kidney tissue culture. Intranasal or intramuscular inoculation of this high passage virus did not cause disease in susceptible cattle. However, intramuscular inoculation of these viruses resulted in a specific antibody response. Thus, the porcine kidney tissue culture-propagated virus can be used as a vaccine, with the additional advantage that such a vaccine would be free of any cattle viruses that might be present in bovine kidney.