Activation of a cellular oncogene by DNA rearrangement: possible involvement of an IS-like element

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Abstract
The cellular oncogene c-mos is rearranged in a mouse myeloma and the tumor mRNA contains transcripts hybridizing with a v-mos probe. The rearranged gene (rc-mos) was cloned in .lambda. phage and shown to transform mouse fibroblasts in transfection assays. rc-mos Differs from its progenitor, c-mos, only at the 5'' end of the gene, where c-mos sequences were substituted by a noval cellular DNA fragment. This fragment contains a 159-base pair (bp) IS-like element localized immediately 5'' to the junction with c-mos. This is the 1st demonstration in a non-virally-induced tumor of activation of a cellular oncogene by a mechanism possibly invovling DNA transposition.