panD, a new chromosomal locus of Salmonella typhimurium for the biosynthesis of β-alanine
- 1 March 1975
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 140 (2) , 159-164
- https://doi.org/10.1007/bf00329783
Abstract
Three mutants of Salmonella typhimurium LT2, which required either pantothenate or β-alanine for growth, were obtained after treatment with N-methyl-N′-nitro-N-nitrosoguanidine. Their phenotype was: SM30 Pan-, SM31 Pan- Met-, SM32 Pan- Thi- (requirement for the thiazole-moiety of thiamine). Neither aspartate, dihydrouracil, nor β-ureidopropionate replaced β-alanine as growth factor. By conjugation it was found that the three genetic lesions (Pan-, Met-, Thi-) were located at about minute 128 of the bacterial chromosome. By transduction 63% linkage was found between the Pan and Met loci, and 84% between the Thi and Pan loci. Probably the thiazole auxotrophy was due to a lesion in the thiG locus. The new genetic locus responsible for the synthesis of β-alanine was named panD.Keywords
This publication has 31 references indexed in Scilit:
- Linkage map of Salmonella typhimurium, edition IV.1972
- F + , Hfr, and F' strains of Salmonella typhimurium and Salmonella abony.1972
- On the process of cellular division in Escherichia coliJournal of Molecular Biology, 1968
- Mutagenesis of the replication point by nitrosoguanidine: Map and pattern of replication of the Escherichia coli chromosomeJournal of Molecular Biology, 1968
- Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in escherichia coli K12Biochemical and Biophysical Research Communications, 1965
- Mutation of Salmonella typhimurium by nitrosoguanidineMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1965
- DIHYDROURACIL AS A GROWTH FACTOR FOR MUTANT STRAINS OF ESCHERICHIA COLIJournal of Bacteriology, 1957
- METABOLISM OF INTERMEDIATE PYRIMIDINE REDUCTION PRODUCTS IN VITROJournal of Biological Chemistry, 1956
- PANTOTHENATE STUDIES .3. DESCRIPTION OF THE EXTRACTED PANTOTHENATE-SYNTHESIZING ENZYME OF ESCHERICHIA-COLI1952
- PANTOTHENATE STUDIES IJournal of Bacteriology, 1950