Desiccation ofPhaseolus vulgarisSeeds During and Following Germination, and its Effect Upon the Translatable mRNA Population of the Seed Axes

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Abstract
Misra, S. and Bewley, J. D. 1986. Desiccation of Phaseolus vulgans seeds during and following germination, and its effect upon the translatable mRNA population of the seed axes.—J. exp. BoL 37: 364–374. After imbibition and germination, seeds of P. vulgaris pass from a stage where they are insensitive to desiccation to a stage where they are sensitive. Desiccation of seeds during the sensitive stage results in an almost total impairment of protein synthesis upon subsequent rehydration. Seeds desiccated during the desiccation-tolerant stage, however, resume protein synthesis at almost control levels. The protein patterns obtained following in Vitro translation of bulk RNA from fresh imbibed, desiccated, and desiccated-rehydrated seed axes were qualitatively similar at 5 HAI (the desiccation- tolerant stage). The drying treatment resulted in increased intensity of extant proteins at 5 and 12 HAI. At 12 HAI (the transition stage between the desiccation-tolerant and desiccation-intolerant phases) desiccation and subsequent rehydration triggered synthesis of a unique set of proteins-the rehydration proteins. At 20 HAI (the desiccation-intolerant stage) desiccation resulted in an overall decline in the intensity of proteins synthesized in vitro. Also the rehydration proteins were not synthesized in response to a drying and rehydration treatment at this time.