Tissue-Specific Distribution of Non-Histone Proteins in Nuclei of Various Tissues of Rats and Its Change with Growth of Rhodamine sarcoma

Publisher Website
Google Scholar
Abstract
1. The total contents of histones and non-histone proteins in the nuclei prepared in the presence of diisopropylfluorophosphate from five kinds of normal tissues and Rhodamine sarcoma of rats were determined, and the species and relative contents of the individual proteins were analyzed by SDS-polyacrylamide gel electrophoresis. 2. In all kinds of tissues examined, the histones were similar in total content, species and relative content. 3. The total content of non-histone proteins varied with the kind of tissue as follows: Rhodamine sarcoma » brain ≥ liver » kidney > spleen ≥ thymus. In each kind of tissue examined, a few species of non-histone proteins were specific, whereas the remaining species were common. However, the relative contents of the individual species varied with the kind of tissue. Most of the tissue-specific non-histone proteins had molecular weights ranging between approximately 30,000 and 40,000 daltons. 4. When Rhodamine sarcoma grew on the back of the animals, the total content of non-histone proteins, but not that of histones, decreased appreciably in the liver. In other tissues, the decrease was insignificant. The decrease in the total content of non-histone proteins in the liver was mostly due to a decrease in a non-histone protein of approximately 52,000 daltons and six non-histone proteins between approximately 30,000 and 40,000 daltons. 5. The tissue-specific distribution of non-histone proteins in all the tissues examined, and its change in the liver with the growth of Rhodamine sarcomajwere not caused by proteases present in the nuclei.

This publication has 1 reference indexed in Scilit: