Identification of cellular proteins modified in response to African swine fever virus infection by proteomics
- 22 June 2004
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 4 (7) , 2037-2046
- https://doi.org/10.1002/pmic.200300742
Abstract
Changes in gene expression are produced in cells as a consequence of virus infections. In the present work, we used proteomic technology to globally examine African swine fever virus (ASFV)-infected Vero cells searching for infection-associated proteins in order to determine target proteins for pathogenesis studies. We studied the alterations in cellular protein profile after ASFV infection by two-dimensional electrophoresis, identifying the modified cellular proteins by matrix-assisted laser desorption/ionization peptide mass fingerprinting. A total of twelve different over-expressed cellular proteins were unambiguously identified. The most significant changes were in redox-related proteins, nucleoside diphosphate kinases, heat shock proteins, members of the Ran-Gppnhp-Ranbd1 complex and apolipoproteins. These cellular protein modifications could represent distinct roles during infection related to apoptosis and transcriptional modulation mechanisms. The present study constitutes the first attempt to understand the dynamics of ASFV-host cell interactions by proteomics.Keywords
This publication has 62 references indexed in Scilit:
- Apolipoprotein E-ε4 protects against severe liver disease caused by hepatitis C virusHepatology, 2002
- Differential regulation of Rb family proteins and prohibitin during camptothecin-induced apoptosisOncogene, 2002
- Proteomic analysis of a developmentally regulated secretory vesicleProteomics, 2001
- Prohibitins act as a membrane-bound chaperone for the stabilization of mitochondrial proteinsThe EMBO Journal, 2000
- Modulation of immune cell populations and activation markers in the pathogenesis of African swine fever virus infectionVirus Research, 1997
- Consumption coagulopathy associated with shock in acute African swine feverArchiv für die gesamte Virusforschung, 1993
- Haemostatic abnormalities in African swine fever/A comparison of two virus strains of different virulence (Dominican Republic '78 and Malta '78)Archiv für die gesamte Virusforschung, 1993
- Diseases associated with HTLV-I: virus, IL-2 receptor dysregulation and redox regulationImmunology Today, 1992
- Cell culture propagation modifies the African swine fever virus replication phenotype in macrophages and generates viral subpopulations differing in protein p54Virus Research, 1992
- Proteins specified by African Swine Fever virus: V.-Identification of immediate early, early and late proteinsArchiv für die gesamte Virusforschung, 1987