Circulating Antibody and Anaphylaxis in Mice

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Abstract
Summary: Using 1.0 mg BSA in incomplete Freund's adjuvant to sensitize mice, and measuring the antibody response as antigen binding per milliliter of sera with the ammonium sulfate salting out technique, experiments were conducted to provide additional information regarding the relationship of circulating antibody to anaphylaxis and the influence of genetic background on both anaphylaxis and the influence of genetic background on both anaphylaxis and the production of circulating antibody. Evidence is presented to demonstrate that: a) C57 and DBA mice produced small amounts of circulating anti-BSA, and were resistant to anaphylaxis with BSA; b) CF1 mice produced more anti-BSA, and were highly sensitive to anaphylaxis; c) the severity of anaphylaxis observed in all three strains appeared related to the amount of antibody present; d) anaphylactic shock was not observed in any strain until circulating antibody could be demonstrated; e) a 2-mg immunizing dose of BSA resulted in an earlier antibody response and anaphylaxis in CF1 mice than did a 1.0-mg dose.