ANTIGENIC MODULATION OF LYMPHOCYTIC SURFACE-IMMUNOGLOBULIN YIELDING RESISTANCE TO COMPLEMENT-MEDIATED LYSIS .1. CHARACTERIZATION WITH SYNGENEIC AND XENOGENEIC COMPLEMENTS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 42  (1) , 7-12
Abstract
The term antigenic modulation is used to describe the induction, by antibody, of resistance to lysis by antibody plus complement [C]. The rapid antigenic modulation in vitro of surface Ig on guinea-pig L2C leukemic lymphocytes is reported; incubation of the cells for 2 min or longer at 37.degree. with anti-Ig diminished or removed completely the lysis occurring during subsequent incubation with anti-Ig plus C. The modulation was effective for both xenogeneic (rabbit) and syngeneic (guinea-pig strain 2) C, but more rapid for the latter. It appeared to require antibody action on a metabolically active cell: no requirement could be demonstrated for any serum component other than antibody, and there was a need to raise the temperature to 37.degree. after antibody attachment. There was molecular specificity inasmuch as modulation with anti-Ig failed to confer any resistance to lysis by another antibody (anti-Ia) plus C.