Effects of trauma on incorporation of l-[35S]cysteine into tissue glutathione

Abstract
Mice whose blood supply to the hind legs was occluded for 3 hr. and which were killed 2.5 hr. after removal of hind-leg ligatures, had appreciably lower concentrations of non-protein SH-group compounds and less incorporation of L-S35 cysteine into glutathione in their tissues, especially liver and kidney, than did controls. Trauma apparently induced decreases in tissue non-protein-SH-group compounds, mainly by inhibiting synthesis of glutathione. This was confirmed by isotopic dilution tests.