Solvent Systems for the Countercurrent Chromatography of Hydrophobic Neuropeptide Analogs and Hydrophilic Protein Fragments

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Abstract
Two-phase solvent systems used in countercurrent chromatography conducted in Ito coil planet centrifuges have been found useful for fractionating solid-phase synthesized cholecystokinin and bombesin analogs. A system composed of chloroform, acetic acid and water has been found to be quite useful for these hydrophobic peptides which are not water soluble. On the other hand, common types of peptides synthesized are protein partial sequences produced for generating antibodies. The most hydrophilic sequences of proteins are usually selected for this purpose. The results of chromatographing a series of such peptides in the n-butanol, acetic acid and water system are presented.