Development of somatic embryos from cell suspension cultures of Norway spruce (Picea abies Karst.)
- 1 March 1988
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 7 (2) , 134-137
- https://doi.org/10.1007/bf00270123
Abstract
Cell suspension cultures were established from embryonal-suspensor masses derived from mature seeds. Transfer of cell masses on to a medium containing abscisic acid stimulated development of already established individual embryos. Somatic embryos developed shoots when supported by cheese cloth in liquid medium in Petri dishes. The percentage of well-developed roots remained low even though all embryos had root meristems. We have recovered an average of 25 plantlets from an initial PCV of ca 1 g fresh wt per 10 ml.Keywords
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