Photoreversal of the Ultraviolet Radiation-Induced Disappearance of ATPase-Positive Langerhans Cells in the Epidermis of Monodelphis domestica

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Abstract
The present study was undertaken to explore the possible causes of ultraviolet radiation (UVR)-induced disappearance of ATPase-positive, epidermal Langerhans cells (LC). Monodelphis domestica was used because it has the capacity for photoreactivation of UVR-induced pyrimidine dimers in epidermal DNA. Single, 330 J/m2 (ears) or 500 J/m2 (back) UVR exposures (FS-40 sunlamps) reduced the numbers of ATPase-positive epidermal LC in M. domestica ears to ~15% of those in unirradiated ears and ~37% of those in unirradiated dorsal skin. Immediate 90-minute exposures to photoreactivating light (PRL, 320–400 nm) post-UVR reversed the effects of the UVR, resulting in ATPase-positive LC numbers not being significantly different from controls. Exposure to PRL immediately proceeding UVR did not prevent ATPase-positive LC disappearance. The photoreactivation of UVR-induced ATPase-positive LC disappearance indicates that DNA damage (pyrimidine dimers) is involved in the loss of ATPase-positive LC.

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