Frozen‐Thawed Mononuclear Cells as a Source of B Lymphocytes for Antibody Screening
- 1 August 1977
- journal article
- research article
- Published by Wiley in Tissue Antigens
- Vol. 10 (2) , 108-113
- https://doi.org/10.1111/j.1399-0039.1977.tb01125.x
Abstract
B lymphocytes were isolated from fresh blood and frozen‐thawed lymphocyte preparations from the same donors. A panel of 55 human antisera, previously found to react with human peripheral blood B lymphocytes in cytotoxicity assays, was screened against these B‐cell preparations. There was essentially no difference in the reactions of B‐cell antisera with fresh B lymphocytes when compared with frozen‐thawed cells. The percentage recovery of B cells from the fresh and frozen preparations was not significantly different.Keywords
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