Stimulation of protein kinase C activity may increase microvascular permeability to colloidal carbon viaα-isoenzyme
- 1 October 1994
- journal article
- Published by Springer Nature in Inflammation
- Vol. 18 (5) , 481-487
- https://doi.org/10.1007/bf01560695
Abstract
The vasculature of the isolated mesentery and small intestine was perfused with a gelatin-containing physiological salt solution in vitro. Various phorbol-related compounds that are known to have different affinities for the protein kinase C (PKC) isoenzymes, and bradykinin (BK), were tested for their ability to cause the microvascular endothelium to become permeable to injected colloidal carbon (CC). Phorbol 12,13-dibutyrate (PDB), 12-deoxyphorbol 13-phenylacetate (DOPPA), thymeleatoxin (TMX), and resiniferatoxin (RFX), each at a concentration of 1μM, were found to increase permeability. Pretreatment with the PKC inhibitor Ro 31–8220 (1μM) significantly reduced the response to all of these compounds. Indomethacin (1μM), on the other hand, reduced only the effect of RFX. 12-Deoxyphorbol 13-phenylacetate 20-acetate (DOPPAA) (1μM) and BK (10μM) did not increase CC leakage. These results suggest that the Ca2+-dependent PKCα-isoenzyme was involved in the increase in endothelial permeability. BK does not appear to stimulate PKC activity in this experimental situation.Keywords
This publication has 22 references indexed in Scilit:
- Lectin-induced increase in microvascular permeability to colloidal carbonin vitro may involve protein kinase C activationInflammation Research, 1994
- Modulation of endothelial autacoid release by protein kinase C: Feedback inhition or non‐specific attenuation of receptor‐dependent cell activation?Journal of Cellular Physiology, 1993
- Involvement of protein kinase C in the control of microvascular permeability to colloidal carbonInflammation Research, 1993
- A Protein Kinase C Agonist, Selective for the β1 Isozyme, Induces E-Selectin and VCAM-1 Expression on HUVEC but Does Not Translocate PKCBiochemical and Biophysical Research Communications, 1993
- The phorbol derivatives thymeleatoxin and 12-deoxyphorbol-13-O-phenylacetate-10-acetate cause translocation and down-regulation of multiple protein kinase C isozymesFEBS Letters, 1993
- The protein kinase C familyEuropean Journal of Biochemistry, 1992
- A phorbol ester and a daphnane ester stimulate a calcium‐independent kinase activity from human mononuclear cellsFEBS Letters, 1989
- Identification of three additional members of rat protein kinase C family: gd‐, ϵ‐ and ξ‐subspeciesFEBS Letters, 1987
- The Complete Primary Structure of Protein Kinase C—the Major Phorbol Ester ReceptorScience, 1986
- The effect of tiaprofenic acid and indomethacin on in vitro prostaglandin synthesis by rat, rabbit and human stomach tissueInflammation Research, 1985