Isolation of a pdxJ point mutation that bypasses the requirement for the PdxH oxidase in pyridoxal 5' -phosphate coenzyme biosynthesis in Escherichia coli K-12
- 1 April 1996
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 178 (8) , 2445-2449
- https://doi.org/10.1128/jb.178.8.2445-2449.1996
Abstract
We isolated 26 suppressor mutations that allowed growth of a delta pdxH::omega null mutant in the absence of pyridoxal. Each suppressor mapped to pdxJ, and the eight suppressors sequenced contained the same glycine-to-serine change in the PdxJ polypeptide. This bypass suppression suggests that PdxJ may participate in formation of the pyridine ring of pyridoxine 5'-phosphate.Keywords
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