SERUM AC-GLOBULIN: FORMATION FROM PLASMA AC-GLOBULIN; ROLE IN BLOOD COAGULATION; PARTIAL PURIFICATION; PROPERTIES; AND QUANTITATIVE DETERMINATION

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Abstract
Plasma Ac-globulin is probably an inert protein or a pro-enzyme. It is changed to serum Ac-globulin by thrombin. Fibrinolysin will not produce the change. Serum Ac-globulin is the active catalyst of the interaction of prothrombin, thromboplastin and Ca ions. It is present in bovine serum and may be formed in oxalated plasma or from purified Ac-globulin by the addition of small amts. of thrombin. Large quantities of thrombin destroy Ac-globulin activity. Thrombin is formed by co-autocatalysis in the blood clotting mechanism. Serum Ac-globulin has been obtained in concentrated form from bovine serum and from defibrinated oxalated bovine plasma. Its chemical properties cannot be differentiated from those of plasma Ac-globulin. Stability studies show that serum Ac-globulin is quite stable in bovine serum. Its activity can be measured quantitatively by the methods described. The function of Ac-globulin in the blood clotting mechanism is summarized by the following equations: Prothrombin + Thromboplastin[image]Thrombin; Plasma Ac-globulin[image]serum Ac-globulin; Prothrombin + Thromboplastin [image] Thrombin; Fibrinogen [image] Serum Ac-globulin Fibrin.