MOLECULAR ANALYSIS OF TWO RECOMBINANT MOUSE STRAINS WITH CROSSOVERS IN THE Eα RECOMBINATION HOT SPOT

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Abstract
Recombination in the E.alpha. gene of the mouse major histocompatibility complex has been found to occur only in mice derived from crosses between strains with the k and p haplotypes. In the present paper the crossover sites of six additional strains were examined by RFLP analysis. These recombinant strains were derived from crosses between strains (B10.RPD1, B10.RPD2) with crossovers in the E.alpha. gene and B10.M (H-2f). Four have crossover sites between the E.alpha. gene and the S region. One recombinant (B10.RFD7) also crossed over within the E.alpha. gene and a second (B10.RPF1) crossed over within a segment located to the left of the E.alpha. gene. This study suggests that the DNA sequence responsible for recombination in the E.alpha. gene is also present in the B10.RPD1 and B10.RPD2 recombinant strains.