Cell mediated PPD specific cytotoxicity against human monocyte targets:

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Abstract
Human Purified Protein Derivative of tuberculin‐ (PPD‐) specific cytotoxic cells have been detected in cultures of peripheral blood mononuclear cells stimulated for 6 days with PPD. These cytotoxic cells are demonstrated by their ability to lyse PPD‐pulsed autologous monocyte target cells, but not unpulsed targets.In a series of checkerboard experiments each involving 3–5 randomly combined donors, effector cells from 35 donors have been tested in autologous and 130 allogeneic combinations. Analysis of results from the pooled allogeneic combinations reveals that HLA‐B — and even more pronounced HLA‐DR — antigen sharing correlates positively to high lysis. No effect of HLA‐A antigen sharing is found. A more detailed analysis shows that the effect of HLA‐B sharing may be fully accounted for by HLA‐B‐DR linkage disequilibrium. The results thus indicate that cell‐mediated PPD specific cytotoxicity is HLA‐restricted. Further, the correlation to HLA‐DR sharing indicates that the restriction element in this system in all probability is a class II antigen.