HLA-D Restriction of the Macrophage-Dependent Response of Immune Human T Lymphocytes to PPD in Vitro: Inhibition by Anti-HLA-DR Antisera

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Abstract
The response of T [thymus derived] cells from sensitized individuals to low doses of PPD [purified protein derivative] in vitro is macrophage-dependent. Testing different allogeneic combinations of macrophages and T lymphocytes showed that an optimal response required that antigen be presented by macrophages sharing at least 1 of the HLA-D determinants of the T cell donor. Antisera recognizing HLA-A, -B or -DR antigens were able to inhibit this proliferative response. The anti-HLA-DR antisera exerted their inhibitory effect only when directed towards an antigen shared by the donors of the T lymphocytes and the macrophages. Anti-HLA-A and -B sera had an inhibitory effect when reactive with the responding T lymphocytes irrespective of their reactivity with the cooperating macrophages. An optimal secondary response of in-vivo-immunized T lymphocytes to PPD probably requires the combined recognition of the antigen and self membrane structures encoded by the HLA-D locus.