Epidermal growth factor: intracellular Ca2+ inhibits its association with pancreatic acini and A431 cells
- 12 December 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 164 (2) , 335-339
- https://doi.org/10.1016/0014-5793(83)80312-3
Abstract
The uptake of 125I‐labeled epidermal growth factor (125I‐EGF) by mouse pancreatic acini was inhibited (40–50%) by the secretagogue cholecystokinin octapeptide (CCK8). Analysis of competitive binding data showed that the apparent K d of EEG binding increased 135% while the binding capacity was only slightly altered (30% increase). That the effect of CCK8 on acini was mediated by intracellular Ca2+ was indicated by the following: (i) Inhibition of 125I‐EGF binding to acini was dose‐dependent and paralleled the known abilities of CCK8, its analogs, and the cholinergic secretagogue carbachol to induce Ca2+ efflux from acini; and (ii) addition of the Ca2+ ionophore A23187 also inhibited 125I‐EGF binding. In addition, EGF association with A431 cells was also inhibited by A23187 in the presence but not the absence of Ca2+.Keywords
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