A catalytic 13-mer ribozyme
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (4) , 1371-1377
- https://doi.org/10.1093/nar/17.4.1371
Abstract
A 13-mer oligoribonucleotide can act as a ribozyme for the specific self-cleavage of a 41-mer oligoribonucleotide substrate in the presence of Mg2+. The two sequences involved correspond to the self-cleavage hammerhead structure of the virusoid of lucerne transient streak virus. The Michaelis-Menten kinetic parameters for there actions were; Km 1.3 .mu.M, Vmax 0.012 .mu.M min-1, kcat 0.5 min-1. The 13-mer RNA is the smallest ribozyme so far reported. A DNA analogue of the 13-mer cannot substitute for the RNA in the reaction.This publication has 16 references indexed in Scilit:
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