The effect of 12-O-tetradecanoyphorbol-13-acetate (TPA) on phospholipid metabolism of human epidermal keratinocytes in culture

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Abstract
The tumor promoter 12- O -tetradecanoylphorbol-13-acetate (TPA) stimulated the release of [ 3 H]choline from prelabelled membrane phospholipids of cultured keratinocytes obtained from normal human skin. In contrast, TPA in the concentration range of 10 -12 to 10 -6 g/ml failed to induce deacylation of [ 3 H]arachidonic acid or stimulate [ 3 H]prostaglandin production in prelabelled keratinocytes. In addition, TPA did not induce [ 3 H]choline incorporation into the membrane phospholipids of these cells. The previously reported inability of TPA to stimulate a proliferative response in these cell cultures may be related to the resistance of these cells to TPA-induced alterations of arachidonate metabolism.

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