DISSOCIATION IN EXPRESSION OF MB1/MT1 AND DR1 ALLOANTIGENS IN MUTANTS OF A LYMPHOBLASTOID CELL-LINE
- 1 January 1983
- journal article
- research article
- Vol. 131 (3) , 1318-1321
Abstract
Cytotoxicity tests with alloantisera were used to study the expression of HLA-D region antigens in HLA-DR-null mutants of a human lymphoblastoid cell line [LCL 721]. The initial cell line contained just 1 copy of the MHC as a haplotype that included DR1 and MB1/MT1. Gamma ray mutagenesis of the single haplotype cells followed by selection with complement and an anti-DR monoclonal antibody were then used to isolate DR-null mutants. Two categories of mutants were identified with a panel of alloantisera. Expressions of DR1 and MB1/MT1 were simultaneously lost in 4 mutants. Nine mutants still expressed MB1/MT1 but had lost the expression of DR1. The dissociated loss of expression of MB1/MT1 and DR1 antigens is evidence for separate genetic control of these alloantigens. The methods used exemplify a versatile approach for conveniently inducing separations of closely linked loci of the MHC [major histocompatibility complex].This publication has 13 references indexed in Scilit:
- Biochemical characterization of a second family of human Ia molecules, HLA-DS, equivalent to murine I-A subregion molecules.The Journal of Experimental Medicine, 1982
- Human B cell alloantigens; alpha subunit variability.The Journal of Immunology, 1982
- Molecular relationships of the human B cell alloantigens, MT2, MB3, MT4, and DR5.The Journal of Immunology, 1982
- Multiple HLA-DR antigens: detection with monoclonal antibodies and translation in vitro.Proceedings of the National Academy of Sciences, 1982
- Human Ia molecules carrying DC1 determinants differ in both α- and β-subunits from Ia molecules carrying DR determinantsNature, 1981
- Human B-cell alloantigens DC1, MT1, and LB12 are identical to each other but distinct from the HLA-DR antigenProceedings of the National Academy of Sciences, 1981
- Two populations of Ia-like molecules on a human B cell line.The Journal of Immunology, 1980
- Gamma ray-induced loss of expression of HLA and glyoxalase I alleles in lymphoblastoid cells.Proceedings of the National Academy of Sciences, 1980
- Identification of a trans-acting function regulating HLA-DR expression in a DR-negative B cell variantSomatic Cell and Molecular Genetics, 1980
- Immunological dissection of human Ia molecules.The Journal of Experimental Medicine, 1978