Response of Erythroid Day 3 Burst-Forming Units to Endotoxin and Erythropoietin

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Abstract
When marrow and spleen cells of plethoric mice were cultured with 1.0 unit/ml of Limulus-lysate-absorbed Ep [erythropoietin], the predominant erythroid progenitor cell assayed was the day 3 BFU-E [erythropoietic burst-forming units]. Erythropoiesis by these cells could be studied by enumeration of bursts at 3 days or by 24-h 59Fe incorporation into heme. Endotoxin markedly increased the number of day 3 BFU-E in spleens of these mice and decreased the number in the marrow but had little effect on erythropoietic maturation. Ep produced differentiation of the erythroid progenitor cells resulting in increased splenic CFU-E [erythroid colony-forming units] and a decline in the more primitive erythroid precursor cells.