The separation of ribonucleic acids from Escherichia coli on lysine-agarose
Open Access
- 1 June 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (6) , 1569-1576
- https://doi.org/10.1093/nar/3.6.1569
Abstract
Lysine-agarose provides a simple means of separating RNA species of different molecular weight. When RNA from Escherichia coli is added to a small column of lysine-agarose and elution is carried out at neutral pH with a shallow linear gradient of NaCl the RNA species are eluted according to size. 4S and 5S RNA species are completely separated and after a delay the 16S and 23S species are eluted as separate peaks. The process is very reproducible and the different species are eluted at a fixed salt concentration even when changes are made in the gradient, provided other conditions, under which the column is run remain constant.Keywords
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