Normal Cells of Patients with High Cancer Risk Syndromes Lack Transforming Activity in the NIH/3T3 Transfection Assay

Abstract
Oncogenes capable of transforming NIH/3T3 cells are often present in human tumors and tumor cell lines. Such oncogenes were not detected in normal fibroblast lines derived from patients with several clinical syndromes associated with greatly increased cancer risk. Thus, germ-line transmission of these oncogenes does not appear to be the predisposing factor responsible for these high cancer risk syndromes.