Transfer of Rabbit Lymph Node Cells to Neonatal Recipient Rabbits

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Abstract
Summary: When lymph node cells were obtained from adult rabbits, incubated in vitro with a filtrate of trypsin-treated Shigella, and transferred to irradiated neonatal recipient rabbits, agglutinins to Shigella appeared subsequently in the sera of these recipients. When approximately the same number of cells was given to neonatal and to adult recipients, the amount of antibody produced per transferred cell was estimated to be about one-fifth as much in the neonatal as the adult recipient. However, with decreasing numbers of cells transferred to the neonatal rabbits there was an increase in the highest relative values of antibody produced per cell until, when the numbers of cells were approximately proportional to the weights of the recipients, the maximal value for the amount of antibody per transferred cell in neonatal recipients was about half of the level in 1-kg recipients. X-irradiation of the neonatal recipients prior to transfer led to higher antibody levels than those found in nonirradiated litter-mates, in the same range of ratios as that found in older recipients. Cells obtained from lymph nodes draining sites of injection of heterologous antigens did not lead to significantly different antibody titers than in the case of cells from uninjected donor rabbits. Neonatal recipient rabbits previously injected with rabbit leukocytes, even on the first day of life, yielded lower agglutinin titers after lymph node cell transfer carried out 1 week later. Complete suppression of the antibody-inducing effect of transferred lymph node cells was found after the injection of 107 leukocytes, and decreasing degrees of suppression in the case of 106 and 105 leukocytes, respectively.

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