FACTORS AFFECTING THE FORMATION OF ACETYLMETHYLCARBINOL BY LACTOBACILLUS ARABINOSUS

Abstract
L. arabinosus 17-5 converts pyruvate quantitatively to CO2 and AMC. The opt. pH for this enzyme system is between pH 3 and 4, and the pH of the culture medium during growth of the organism must reach that required for opt. activity before max. enzyme formation occurs. Vacuum-dried cells retain their ability to convert pyruvate to CO2 and AMC under the same conditions found for resting cells. Such prepns. decarboxylate alpha-acetolactic acid to CO2 and AMC. The reported stimulation of this system by glucose apparently is due to a permeability barrier to pyruvate in intact cells of L. arabinosus.