Comparative studies of in vitro transformation by ethylene oxide and gamma-radiation of C3H/10T1/2 cells

1 January 1989

journal article
research article
Published by Oxford University Press (OUP) in Mutagenesis

Vol. 4 (1) , 58-61
https://doi.org/10.1093/mutage/4.1.58

Abstract

A dose-dependent transforming ability of the direct-acting alkylating agent, ethylene oxide (EtO), was demonstrated in C3H/10T1/2 mouse embryo fibroblasts. Morphologically transformed colonies were observed 5–6 weeks after the treatment with EtO. The transforming effectiveness of EtO was compared with that of gamma-radiation, and the rad-equivalence of EtO was calculated to be ∼ 90 rad/mMh. This value is consistent with earlier estimates based on induced mutation in various biological systems and thus indicates the applicability of the C3H/10T1/2 cell system in the risk estimation of human exposure to genotoxic compounds.

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