IMMUNOHISTOCHEMICAL LOCALIZATION OF ALPHA-1-ANTITRYPSIN IN NORMAL MOUSE-LIVER AND PANCREAS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 101  (3) , 723-+
Abstract
Using horseradish peroxidase and fluorescence immunohistochemistry, .alpha.1-antitrypsin (.alpha.1AT) was demonstrated in normal mouse hepatocytes and pancreatic islet cells. All hepatocytes were positive; 1-3% stained intensely for .alpha.1-AT. These were located mainly in the periportal area as well as randomly distributed, singly and in clusters, throughout the liver lobule. Nonparenchymal liver cells were negative for .alpha.1AT. The type of hepatocyte cytoplasmic staining appears to alter during ontogeny, changing from a localized granular to a diffuse pattern. The use of immunohistochemistry to demonstrate .alpha.1AT in normal mouse liver allows us to examine the acute phase response at a cellular level.