PROTECTION AGAINST PNEUMOCOCCAL SEPSIS IN SPLENECTOMIZED RATS BY IMPLANTATION OF SPLENIC TISSUE INTO AN OMENTAL POUCH
- 1 January 1982
- journal article
- research article
- Vol. 91 (6) , 638-641
Abstract
This study evaluates the effect of splenic autotransplantation into an omental pouch on pneumococcal infection in rats. Healthy, young Sprague-Dawley rats were divided into 3 groups of 40 each. Group 1 served as anesthetized sham-operated controls. Group 2 had a splenectomy. Group 3 had a splenectomy with autotransplantation of 2 slices of spleen 3 mm thick into surgically created omental pouches. At 16 wk after surgery, all animals were inoculated with Streptococcus pneumoniae type 25 in a dose of 2 .times. 107 (LD50 for splenectomized rats). At 14 days after inoculation, all group 3 survivors were killed and the transplants were removed for examination. Group 1 had 100% survival rate following bacterial inoculation. There was a significant difference in survival rate between group 2 (56%) and group 3 (89%) (P < 0.001). Pathologic examination of group 3 demonstrated hyperplastic regeneration of splenic tissue within the omental pouch. Rats bearing reimplanted splenic tissue in an omental pouch were protected against pneumococcal peritonitis.This publication has 4 references indexed in Scilit:
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