INVIVO STUDIES ON THE REGENERATION KINETICS OF ENRICHED POPULATIONS OF HEMATOPOIETIC SPLEEN COLONY-FORMING CELLS FROM NORMAL BONE-MARROW

  • 1 January 1983
    • journal article
    • research article
    • Vol. 16  (4) , 385-392
Abstract
Functional properties of mouse hemopoietic spleen colony-forming cells, enriched 40- to 80-fold, from normal bone marrow were studies. The number of partially purified CFU-s (colony forming unit-spleen) required to rescue lethally irradiated mice was similar to the number of normal unfractionated bone marrow CFU-s giving the same level of protection; the homing of partially purified CFU-s was similar to that of CFU-s from unfractionted bone marrow; the regeneration of CFU-s in spleen was similar for enriched and unfractionated cell populations between 4 and 11 days after transplantation. In contrast, the rate of regeneration of CFU-s in femur was slower with enriched progenitor cells than with unfractionated bone marrow. The growth rate in femur, however, could be restored to normal by injecting freshly isolated syngeneic thymocytes with the enriched CFU-s population. The partially purified CFU-s are by themselves functionally normal and show that the rate of CFU-s repopulation in bone marrow can by affected by cell types other than CFU-s.