The separation and identification of small amounts of mixed amino sugars

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Abstract
A method is described for the isolation and characterization of small quantities (10-20 mg.) of glucosamine and chondrosamine when these sugars are found together and in the presence of other sugars and amino acids, as in the human blood-group mucoids. The method depends on the conversion of the amino sugars into their N-2,4-dinitro-phenyl derivatives and the chromatographic separation of these substances using alkaline borate as the aqueous and 30% (v/v) amyl alcohol-chloroform as the organic phase. The separation is probably due to the formation of a complex between the cis-diol of the dinitrophenyl derivative of chondrosamine and the metaborate ion.
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