Demonstration of serotonin receptors in isolatedSpisulaoocyte membrane
- 1 February 1991
- journal article
- research article
- Published by Taylor & Francis in Invertebrate Reproduction & Development
- Vol. 19 (2) , 147-150
- https://doi.org/10.1080/07924259.1991.9672168
Abstract
A method for the isolation of Spisula oocyte membranes was developed. 5-hydroxytryptamine (5-HT) receptors in the isolated oocyte membranes were determined by measuring the binding of [3H]5-HT. The dissociation constant of [3H]5-HT binding to the membrane receptor was estimated to be 17.5 nM and the maximum binding capacity was 7.9 pmoles/mg protein. Inhibition study of [3H]5-HT binding with site selective 5-HT analogs was performed. The results suggest that the 5-HT receptors in oocyte membranes contain sites that interact with 5-HT1 and 5-HT3 selective analogs.Keywords
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