Growth of spiroplasmas in the presence of various nutrients and metabolic inhibitors

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Abstract
Glycolytic intermediates replaced glucose as carbohydrate sources in a chemically defined medium CC-494N, whereas keto acids of citric acid cycle intermediates and glyoxylate were supplemented with 10 mM glucose. Glucose-6-phosphate, fructose-1,6-diphosphate, 3-phosphoglyceraldehyde, and pyruvate supported limited growth for Spiroplasma floricola (23-6), whereas only pyruvate supported SR 3 spiroplasma and Spiroplasma melliferum (AS 576). Oxalacetate and glyoxylate inhibited growth at 10 mM or greater. Hematin, 2,4-dinitrophenol, sodium fluoride, and arsenite were dramatically inhibitory, whereas acetic anhydride, tricarballylate, acetylene dicarboxylate, and sodium cyanide were mildly inhibitory at 10 mM or lower. Malonate at 50 mM was not inhibitory.