Cloning and functional expression of a cardiac inward rectifier K+ channel
Open Access
- 24 January 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 338 (1) , 107-111
- https://doi.org/10.1016/0014-5793(94)80126-6
Abstract
We have isolated a cDNA coding for an inward rectifier K+ channel (RBHIK1) from rabbit heart. The cloned cDNA encodes a protein of 427 amino acids with two putative transmembrane segments. The primary structure of RBHIK1 is highly homologous to that of IRK1 which is an inward rectifier K+ channel recently cloned from mouse macrophage by expression cloning. When expressed in Xenopus oocytes, RBHIK1 current showed strong inward rectification and was inhibited by extracellular Ba2+ and Cs+. RNA blot analysis revealed the expression of RBHIKl mRNA in various rabbit tissues, especially high level in the ventricular muscle.Keywords
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