Ribosomal protein L16 binds to the 3′‐end of transfer RNA
- 23 January 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 166 (1) , 53-56
- https://doi.org/10.1016/0014-5793(84)80043-5
Abstract
Escherichia coli 50 S ribosomal subunits were reconstituted with and without protein L16 present. The latter particles, although active in puromycin reaction, were unable to use CACCA-Phe as an acceptor substrate. We also found that L16 interacts directly with this oligonucleotide and, in the complex with tRNA, protects its 3'-end from pancreatic ribonuclease digestion. We suggest that the role of L16 is in the fixation of the aminoacyl stem of tRNA to the ribosome at its A-site.Keywords
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