Freeze‐fracture of 3T3 cells for high‐resolution scanning electron microscopy
- 1 June 1988
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 150 (3) , 211-218
- https://doi.org/10.1111/j.1365-2818.1988.tb04639.x
Abstract
Triton-extracted, freeze-fractured 3T3 cells have been examined in the Hitachi S-900 field-emission SEM, after light platinum coating, at low beam voltage to evaluate the performance of the microscope under these conditions. For unstained material fixed in glutaraldehyde alone, high-resolution images can be obtained, at accelerating voltages of 1.5-5 kV, after rotary deposition of platinum to an average thickness of 1.5-3 nm. Comparisons are made between these results and those of studies by TEM of deep-etch replicas of similar material previously published.Keywords
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