High Performance Liquid Chromatographic Determination of Dihydrostreptomycin Sulfate in Kidney and Meat Using Post Column Derivatization

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Abstract
A high performance liquid chromatographic method for the determination of dihydrostreptomycin sulfate in kidney and meat from cow, and swine, has been developed. The sample was treated with trichloroacetic acid, and the supernatant clean-up was performed using a Bond Elut Certify II pretreatment column. The lower limit of quantification was 40ng/g and the limit of detection close to 20ng/g. The recovery of dihydrostreptomycin sulfate varyied from 73.2 to 73.5% and from 80.0 to 82.9% for kidney and muscle, respectively.