Poliomylitis. IV. Some Cultural and other Characteristics of Chick-Embryo-Adapted Type II Strain of Poliomyelitis Virus

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Abstract
Effects of incubation time and temp., age of embryo, and route of inoculation on multiplication of a Type 2 chick-embryo propagated poliomyelitis virus were studied. The distribution of the virus in chick embryo tissues and fluids, and its action on the hatchability of infected embryos, were investigated. The virus was inoculated into 6-to 9-day-old embryos, via either the allantoic cavity or the yolk sac, and incubated for 4-6 days at 35[degree]-37[degree]C. The best source of the virus was the embryo proper. No greater concn. of virus was found in the head than in the body. Although the embryos seemed to overcome the infection by the 12th or 13th day, relatively few hatched. The majority died between the 22nd and 27th day of total incubation. The mouse LD50 titers of the chick-embryo-adapted virus were essentially unaffected by 9 consecutive freezings and thawings of the same virus prepn.